> From: DoranC@AOL.COM > Date: Thu, 23 Oct 1997 17:07:44 -0400 (EDT) > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: cell sorting > > I have recently been trained on the FACS Vantage and was told that while > sorting cells, the abort rate should not be greater than 10% of the event > rate (sys. threshold). My problem is that the abort rate is running approx. > 20 - 30%. I acquire events usually around 2000, and most often sort > normal-R, 3 drops. I have tried to alter the concentration, slow down the > rate, and even filter the sample thru a filter cap. Any ideas? > What I have learned working with the Vantage ist that proper optical alignment (i.e. y-knob, focussing the laser beam, and optimizing the fluorescence sigmals) helps in reducing abort rates; also it is crucial to have the dead time adjust knob on card cage slot 13 set properly. Play with that knob a little while running a sample and monitoring the abort rate. Good luck, marcus. ______________________________________________ Dr. Marcus Reckermann Research and Technology Center Westcoast (FTZ) Hafentoern, D-25761 Buesum, Germany E-Mail recker@ftz-west.uni-kiel.de Tel. +49-(0)4834-604-204 or -212 or -261 Fax. +49-(0)4834-604-299
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