I have recently been trained on the FACS Vantage and was told that while sorting cells, the abort rate should not be greater than 10% of the event rate (sys. threshold). My problem is that the abort rate is running approx. 20 - 30%. I acquire events usually around 2000, and most often sort normal-R, 3 drops. I have tried to alter the concentration, slow down the rate, and even filter the sample thru a filter cap. Any ideas?
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:17 EST