We are looking at performing quantitation of D positive cells (FMH) by flow cytometry. I have been trialing Fluro-D (FITC labelled, CSL product) using the method as recommended by the manufacturer. So far I have found that my standard curve results (O Neg blood spiked with O Pos blood) don't always correlate as close as I would expect/like. Is this due to poor technique or have other operators found similar results. So far, I have been able to detect low levels of D positive cells with minimal background. However, I would appreciate any comments from other users about potential pitfalls, correlation to Kleihauer results, best method of reporting (% or mls of D cells) and any other general hints. Thanks to all replies, Wanda
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