Has anyone used flow cytometry for examining oocytes? I am after information on effective nuclei isolation from eggs with a hatching envelope that resists detergents. I am looking at the use of enzymes to remove the envelope and I was wondering if anyone knew of a protocolto help me on my way. There are also a number of other questions I am hoping to ask someone. Thanks, -------------------------------------- Neil Young Research Technician ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Australian Institute of Marine Science PMB No. 3 Townsville MC, Q. 4810 ph: (077) 534308 fax: (077) 725852 email: n.young@aims.gov.au ======================================
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