Could you please post the following message: For some time we have been examining Annexin V- FITC (+ - EDTA) binding. PI uptake in elutriation purified cryopreserved human monocytes cultured in low adherence plates and have observed high Annexin V binding at Zero-time incubation (6o% of the cells) (PI 3-5%). After a short time (less than 4 hours) Annexin V drops to about 15% (PI 3-5%) Similiar values for Annexin V and PI are observed after 24 hours incubation. Recovery of cells from the wells is excellent (manuel counting in counting chamber- better than 9o % at all harvesting times). We wonder if cell-membrane pertubation (leading to Annexin V binding ) may be reversible. In other words may "flip-flop" bee reversible or should it always lead to apoptosis?? Appreciate any answer/suggestion. Thanks Peter Kierulf,MD PhD. Professor I Clinical Chemistry Dep. Ulleval University Hospital N-0407 OSLO, NORWAY Telephone: +47 22 11 94 86 Telefax: +47 22 11 81 89 _-----------------------------------Mobil telphone: 922.85111 E-mail (RFC Formate): peter.kierulf@ioks.uio.no E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no Peter Kierulf,MD PhD. Professor I Clinical Chemistry Dep. Ulleval University Hospital N-0407 OSLO, NORWAY Telephone: +47 22 11 94 86 Telefax: +47 22 11 81 89 _-----------------------------------Mobil telphone: 922.85111 E-mail (RFC Formate): peter.kierulf@ioks.uio.no E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no
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