Robert I have had not a problem compensating PECy5 and PE on our MoFlo. I can't tell from your description what exactly you think is wrong. If you can elaborate maybe we can "sort" it out. Larry Arnold Univ. of North Carolina at Chapel Hill At 05:27 PM 10/21/97 +1000, you wrote: > >Dear Flowers, > >I have been using Winlist to compensate PeCy5 versus Pe labelled antibodies >(CD34-PECy5/CD38-PE) using listmode files and have found it rather difficult >to set up the system correctly (using cells labelled with single >fluorochromes). The position of the regresion line used to compensate will >influence the proportion of double (CD34+CD38hi) and single positive cells >(CD34+CD38lo). Similarly we have had problems using a Moflo sorter to >compensate (in software) before setting sort gates. This is particularly >annoying as we would like to take full advantage of the multiparameter >machine. Is this most likely a software or fluorochrome spectral overlap >problem? Any advice? > > >Many thanks in advance. > > >Robert Nordon >Post Doctoral Fellow >Graduate School of Biomedical Engineering >University of New South Wales >Sydney, 2052 >Tel. no. 61-2-9385-1148 >Fax 61-2-9663-2108 > >
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