DNAse and cell clumping

From: Ralph Bohmer (ralph.bohmer@es.nemc.org)
Date: Tue Oct 21 1997 - 08:55:33 EST 

According to the textbooks, DNAseI makes single-strand nicks in the presence 
of Mg++ and double-strand breaks in the presence of Mn++ (10mM). However, to 
prevent cell clumping, everybody appears to be using DNAse in plain PBS 
(probably Ca++ and Mg++ -free).
Mn++ gets never mentioned. 
To prevent or undo cell wrapping by DNA, don't we want double-strand breaks ?

Ralph M. Bohmer, New England Medical Center

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