>Is anyone on this mailing list involved in PBSC / BMT procedures, if so I
>would like to hear from them?
Dear Dr. Yousaf:
I'm involved in PBSC/BMT, serving as the medical director of the Cell
Therapy Clinical Laboratory at University of Minnesota. This laboratory
performs cell processing, or graft engineering, of the marrows, PBSCs, and
cord bloods transplanted here.
You did not indicate your specific interest -- I hope I may take the liberty
of telling you a bit about what we do. In no particular order:
Graft cryopreservation -- marrow, PBSC, cord blood
Positive selection for CD34+ cells, and for CD34+/HLA-DR- cells from
marrow and PBSC
Production of stroma-conditioned medium for stem and progenitor cell
expansion
Transduction and ex vivo expansion of CD34+/HLA-DR- cells --
currently for patients with CML
Transduction and expansion of lymphocytes for Hunter's disease gene
therapy
Autologous natural killer cell activation and expansion -- for graft
vs. tumor effect
T-cell depletion by counterflow elutriation -- allogeneic marrow
T-cell depletion by CD34 positive selection with CD2 negative
selection -- allogeneic PBSC
RBC depletion for major ABO-incompatible marrow transplants
Plasma removal for minor ABO-incompatible marrow transplants
Coming in the near future are:
Transduction and expansion of CD34+ cells for Hurler's syndrome gene
therapy (in scale-up at present)
Allogeneic natural killer cell activation and expansion -- for graft
vs. tumor effect
Transduction of T-cells with the cytosine deaminase "suicide" gene
and a gene for a selectable cell-surface molecule, followed by selection
and expansion.
Positive selection for umbilical cord blood CD34+ cells, followed by
tansduction and expansion.
Could you let me know what your interest is? Looking at the list above, I
suspect it's more than you wanted to hear -- now you have a chance to do the
same!
Scott
***************************************************************
Scott R. Burger, M.D.
Medical Director, Cell Therapy Clinical Laboratory
Department of Laboratory Medicine and Pathology
University of Minnesota
burge009@gold.tc.umn.edu
612-626-4919
612-625-2199 (Fax)
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