> We are attempting to produce a population of "dead" WBC to spike into > a live dead assay and need to find a way to kill the cells without > destroying the CD14 marker. Dear Dr. Koratich: We've used a brief (10 minute) incubation at 65oC to kill hematopoietic progenitor cells for a similar purpose -- generating a standard curve of viable/dead cells for assay validation. I've never looked at expression of CD14 on these cells, but the method does kill cells while leaving them structurally intact. Scott ----------------------------------------------- Scott R. Burger, M.D. Medical Director, Cell Therapy Clinical Laboratory Department of Laboratory Medicine and Pathology University of Minnesota burge009@gold.tc.umn.edu 612-626-4919 612-624-5411 (Fax)
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