RE: Cell killing and CD markers

From: Scott R. Burger (burge009@gold.tc.umn.edu)
Date: Wed Oct 15 1997 - 12:33:56 EST


>     We are attempting to produce a population of "dead" WBC to spike into 
>     a live dead assay and need to find a way to kill the cells without 
>     destroying the CD14 marker.  

Dear Dr. Koratich:

We've used a brief (10 minute) incubation at 65oC to kill hematopoietic 
progenitor cells for a similar purpose -- generating a standard curve of 
viable/dead cells for assay validation.  

I've never looked at expression of CD14 on these cells, but the method does 
kill cells while leaving them structurally intact.

Scott



-----------------------------------------------
Scott R. Burger, M.D.
Medical Director, Cell Therapy Clinical Laboratory
Department of Laboratory Medicine and Pathology
University of Minnesota
burge009@gold.tc.umn.edu
612-626-4919
612-624-5411 (Fax)



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