Re: GFP, Fixation?

From: Simon Monard (Simon_Monard@adarc.org)
Date: Tue Oct 07 1997 - 09:40:26 EST

Next message: David Chambers: "Re: GFP, Fixation?"
Previous message: Derek Davies: "Re: GFP, Fixation?"
Maybe in reply to: Simon Monard: "GFP, Fixation?"
Next in thread: David Chambers: "Re: GFP, Fixation?"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Sorry missed your question at the end of your reply

If you want to do surface staining with PI then staining with surface
antibodies, fixing for 30 minutes with PFA then ethanol fixation works OK.
If you fix in PFA much longer the CVs are rubbish. I've only used FITC with
bright T-Cell markers.

Simon
ADARC
NYC

Next message: David Chambers: "Re: GFP, Fixation?"
Previous message: Derek Davies: "Re: GFP, Fixation?"
Maybe in reply to: Simon Monard: "GFP, Fixation?"
Next in thread: David Chambers: "Re: GFP, Fixation?"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:12 EST