GFP, Fixation?

From: Simon Monard (Simon_Monard@adarc.org)
Date: Mon Oct 06 1997 - 14:45:04 EST

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Hi All

Simple question: Does formaldehyde fixation affect GFP fluorescence? I've
seen some protocols involving fixing in 4% formaldehyde for an hour or more
then washing cells in PBS before running on the cytometer, surely
unnessesary? Can GFP leak out of fixed cells?

Thanks

Simon Monard
ADARC

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