We have used the Dako flow products for several years and find them to be excellent. We are in the process of setting up a demo with the new "Medimachine". I must say, our experience with any of the so called "prep" machines, has not been productive. However, being the eternal optimist, I would really like to find a DNA "mincer" that could save me from a future carpal tunnel surgery. Kathy Altig PPMC ______________________________ Reply Separator _________________________________ Subject: DAKO products experiences Author: ,Robert Pyle [SMTP:pyle@smtpgw.kfshrc.edu.sa] at PHSOR Date: 10/1/97 8:33 AM I have just had a visit from our local DAKO salesman. He presented me with a new catalog and some advertisement information. Since DAKO is more popular perhaps in immunocytochemistry than it is in flow cytometry, their prices for their antibodies (single color, dual color and tri-color mixes) were much more attractive than the top three or four antibody manufacturers that we normally use for flow cytometry. This leads me to ask two questions: 1. For those flowers out there using DAKO products, what have been your experiences? Do the antibodies perform up to the standards of other manufacturer's? How does their fluorochrome performance compare? What prompted you to choose DAKO over other name brands? 2. Is there anyone out their using their new "Medimachine System" for standardization in the preparation of cell suspensions from solid tissues. At first glance, it looks pretty neat but I am not convinced about the cost effectiveness or performance. If anyone can comment on either I would be very interested. We do a lot of solid tissue analyses and it might be a real boon as a standardized approach to disaggregation. Any and all comments are welcomed and appreciated. Haywood Pyle King Faisal Specialist Hospital and Research Center Flow Cytometry Core Facility MBC #3, Box 3354 Riyadh 11211 Saudi Arabia e:pyle@kfshrc.edu.sa
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