hi again-- this isn't a flow question, but you all seem to know where the antibodies are! i've got a colleague who needs to show (by western blot) that his nuclear fractions aren't contaminated with cytosolic proteins. i suggested looking for ribsomes, ubiquitin or cytosolic beta-glucosidase or using a fluid phase marker. do you have any suggestions as to what he can use, or where to find the antibodies against these proteins? thanks (in advance for this one and belatedly for the last.) barbara ****************************************************************************** Barbara A. Butcher, Ph.D. James A. Baker Institute for Animal Health Hungerford Hill Road Cornell University College of Veterinary Medicine Ithaca, New York 14853 (607) 256-5647 Lab (607) 256-5618 Office (607) 256-5608 FAX **************************************************************************** **
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