To throw in my two cents worth: I have been involved with flow cytometry since 1986 and during that whole period have used, and have instructed the technicians I have taught to use, 2.5% calf bovine serum in PBS for washing and final resuspension of samples to be analyzed. Of course, we have gotten consistently good results doing this, or we wouldn't have continued to do it. We normally get tight population distributions, whether looked at as contour or dot plots or as histograms, unless other factors such as instrumental or cell sample quality problems are present. Our printouts are "publication quality" and appear in many journal articles. Kevin G. Waddick, Ph.D. Staff Scientist Hughes Institute 2685 Patton Road Roseville, Minnesota 55113 (612) 628-0228 ext. 209 (612) 628-9891 Fax
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