Cross-linked anti-IgM

From: Ronald Rabin (RRABIN@atlas.niaid.nih.gov)
Date: Wed Sep 24 1997 - 16:38:08 EST

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How about an anti-IgM that is biotin-conjugated.  Then, just add avidin,
and that will cross link quite well.  Certainly works with anti-CD3 mAb.



Hi Everyone,

I've been looking for a source of cross-linked anti-human IgM to use in
B
cell activation studies. We were getting a reagent (Rabbit
anti-human-IgM
conjugated to beads) made by BioRad, but they no longer make it. Does
anyone
know of a supply of anti-human IgM linked to Dextran or Ficoll or beads
that
is suitable for in vitro culture.

Many Thanks

Pete

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