Simon Monard writes: Hi folks Does anyone know of a way of identifying plasma cells by flow? I have CD38 but it doesn't seem very specific. We wish to monitor the differentiation of primary B-cells into plasma cells. ==================== Hi Simon, We have used B-B4 monoclonal antibody made from a myeloma cell line. It appears quite specific, ie positivity in plasma cell dyscrasias, and have not noticed any positivity when tried on B- NHL, or other lymphoid malignancies. Our commercial source is Serotec Canada, 1-800-265-7376. Can try the internet address to obtain a closer contact (www.serotec.co.uk). The order # for PE conjugated is MCA681PE, and for FITC conjugated the # is MCA681F. Hope this helps. Paul Harris Flow Cytometry Lab St Joseph's Health Centre London, Ont. ph (519) 646 6100 ext 5918 email paulh@stj.stjosephs.london.on.ca
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