I have used this technique with good signal amplification using streptavidin labeled fluorescent microspheres. Once you can block the non-specific binding by the microspheres they really provide a nice signal. We presented a poster on the use of Molecular Probes's streptavidin beads for signal amplification at ISAC in 1996. You can also take a look at: Biochim. Biophys. Acta 1983, 728, 112-20 Analytical Biochem. 1994, 222, 441-9 KUKURUGA@medmail.med.umich.edu wrote: > I've heard of a technique for amplifying signal of low density > antigens by > creating layers of avidin-biotin complex, thereby increasing the > available > biotin targets for the finally added fluorescent-conjugated avidin. > Anyone > know about this, and do you have references? I've searched, but I > guess > I can't come up with the proper key words. > Thanks. > MAK. > > --- > Mark A. KuKuruga, Managing Director > University of Michigan Flow Cytometry > kukuruga@medmail.med.umich.edu -- -------------------------------------------------------- Mahesh K. Bhalgat, Ph.D. Staff Scientist and Flow Cytometry Products Manager Molecular Probes, Inc., OREGON Phone: 541-465-8300 Fax: 541-344-6504 Email: mahesh@probes.com --------------------------------------------------------
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