If there is any discrepancy between what I wrote and what was written by any of my esteemed colleagues, especially Dr. Shapiro, I would stick with whatever THEY wrote. I'll send a subsequent message with all the replies to my original question. NB, thanks to everyone for their suggestions. Rafael Nunez wrote: > Dear Flow-ers > We (Rafael and Claudia) would like to perform some apoptosis assays in > > cells that have been stably transfected with an inmediate early gene > from > Bovine herpes that is under the control of the tetracyclin operon. > Therefore, we would like to get more information about how reliable > are the > uptake of "Hoechst 33258 and 33342" and "7-AAD" for apoptosis, a few > days > ago similar question was asked "Who has expeience using Hoechst uptake > as > an indicator of apoptosis?". At the moment, the comments from Miller > and > Shapiro seems a little bit controversial, is it not?. We are doing our > > homework but your comments will be very much appreciated. > Thank you for the help. > Rafael Nunez MD.
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