> I am looking for protocol(s) regarding the conjugation of PE to a mouse >anti-human IgG monoclonal antibody. I have one protocol from Current >Protocols in Cytometry. I am hoping to find someone who has done this >before, for those helpful hints, ect. >Thank you, >Miguel Suderman I'm sure you will plenty of answers to this, but here goes- There is an excellent protocols page by Mario Roederer, although I haven't tried the PE method myself- http://cmgm.stanford.edu/~roederer/abcon/ Also Prozyme has released a kit which sounds relatively easy to use and although it seems slightly expensive it is probably good value compared with buying conjugates etc., and may be more reliable than starting from scratch- http://www.prozyme.com/phycobiliproteins/ I have been sent the method sheet and it seems straightforward. They also sell activated SMCC-PE separately to the kit. ______________________________________________ Dr William Smith Institute for Child Health Research (Company Limited by Guarantee ACN 009 278 755) Subiaco, Western Australia, 6008 PO Box 855 West Perth WA 6872 Ph 61 8 9340 8792/8388, Fax 61 8 9388 3414 email williams@ichr.uwa.edu.au ______________________________________________
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:05 EST