Also relevant, is the observation of the effect of emission difference on compensation that Joe Trotter illustrated some time back. See the FITC/Fluorescein Alert! at: http://facs.scripps.edu/cd4fitc.html Dave dcoder@u.washington.edu >Hi folks, >While I'm certainly no expert in this area, I feel compelled to >point out that it is the fluorescence _intensity_ of fluorescein >that varies with pH; the peak emission wavelength does NOT change. See >Dr. Haugland's chapter in the latest Methods in Cell Biology manual (part >B), p 658. I had to rebut this point in response to a reviewer's >comments recently, which is the only reason I remember it ;-) > >I have seen the same spectral difference with an antibody made here on >campus, but the details of its conjugation were not available. In this >case I suspect Dr. Prussin is on the right track; it's a matter of >FITC =//= fluorescein. > >Regards, >Steve > >--------------------------------------------------------------- >Steve G. Hilliard steve@habanero.cb.uga.edu >Cell Analysis Facility, University of Georgia >If you're a runner you should be dead. >http://storm.cadcam.iupui.edu/drs/drs.html >--------------------------------------------------------------- >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:05 EST