Hi folks, While I'm certainly no expert in this area, I feel compelled to point out that it is the fluorescence _intensity_ of fluorescein that varies with pH; the peak emission wavelength does NOT change. See Dr. Haugland's chapter in the latest Methods in Cell Biology manual (part B), p 658. I had to rebut this point in response to a reviewer's comments recently, which is the only reason I remember it ;-) I have seen the same spectral difference with an antibody made here on campus, but the details of its conjugation were not available. In this case I suspect Dr. Prussin is on the right track; it's a matter of FITC =//= fluorescein. Regards, Steve --------------------------------------------------------------- Steve G. Hilliard steve@habanero.cb.uga.edu Cell Analysis Facility, University of Georgia If you're a runner you should be dead. http://storm.cadcam.iupui.edu/drs/drs.html ---------------------------------------------------------------
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