hello again: i've been following endocytosis (using dextran-labeled texas red) of a glycoprotein (detected by FITC immunofluorescence) with confocal microscopy. now i am interested in determining if the glycoprotein is increasing the endocytic activity of my cell line. i think i will be able to do this using a facscalibur (i.e., by looking for an increased fluorescence intensity due to dextran uptake). the texas red isn't compatible with the 488 line. can anyone suggest a conjugate that would be appropriate (and not pH sensitive, as i do not want to lose signal in late endosomes)? also, who, besides molecular probes, carries fluorescent dextrans? as always, i appreciate your help. cheers, barbara butcher ****************************************************************************** Barbara A. Butcher, Ph.D. James A. Baker Institute for Animal Health Hungerford Hill Road Cornell University College of Veterinary Medicine Ithaca, New York 14853 (607) 256-5647 Lab (607) 256-5618 Office (607) 256-5608 FAX **************************************************************************** **
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:04 EST