Dear All,
Saw a weird one yesterday - a routine 3/4/8/19/45 screen on a
suspected Kala Azar (aka - Leishmaniasis) that appeared to show the normal
B cells picking up FITC conjugates (by one decad more than a normal
specimen) but not PE or PE-CY5.
This occured with IgG1, 2a & 2b classes, whether isotypic or specific.
The bone marrow I ran today showed it was actually a Pre-B leuk which
fitted into the 5% 45weak/19+ cells I picked up in the peripheral screen.
The main point is - this FITC binding with the (normal & leukaemic) B cells
had disappeared when processing the BM, probably due to the washing steps.
Obviously it's something in the plasma of this kid - but what ?
I've never seen anything "specifically" picking up FITC before
Any Ideas
Wal Sharp
SQU
Oman
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