>I use a FACScan along with Simulset software to enumerate T- lymphocyte subsets >on HIV positive patients. On a few occasions I have seen two distinct lymphocyte populations. >On gating these populations, whether individually or together, the T+B+NK cells are much >lesser than the ideal 100%. >Can someone give me some ideas as to why this is happening and how I can account >for the 'missing' cells. >Thank you . >Cheryl Palmer Cheryl, I have seen this sort of thing a few times and it has always been in the context of a T cell lymphoproliferative disorder. The malignant clone of cells having a lower expression of Eg: CD3; I saw a very nice example recently - a case of Sezary Syndrome with a separate population Of CD3+, CD4+ lymphoid cells which had distinctly less CD3 than the main group of T helpers (which had precisely the same level of CD3 expression as the CD3+, CD4- population on the CD3/CD4 bivariate plot. Flow is regarded as *usually not helpful* in T cell disorders - but in this case it was nice to be able to accurately determine the number of Sezary cells in the PB Do your patients have known/suspect lymphoproliferative disorders ?? Regards Peter Chapple Melbourne AUSTRALIA
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