Re: Commercial QC Products for Leukemia/Lymphoma Immunophenotyping?

From: PLM Enterprises and Designs (plem@erols.com)
Date: Wed Aug 06 1997 - 13:40:48 EST


Theresa,

Try contacting the ATCC...sorry, don't have the address, but they are a 
bank of thousands of cell lines from which you can purchase.

(A'flowin' just down the road from you!......)

P. Echeagaray
Flow Cytometry Services
Southern Research Frederick
Frederick MD
echeagaray@sri.org

Theresa L. Fecik wrote:
> 
> Hi all,
> 
> I'm a newbie here on this board--though, not necessarily a newbie in flow
> cytometry.  I have about 10 years experience in flow--but, I have also
> been working in other areas of the lab at the same time, i.e.,
> hematology, coagulation, urinalysis, and special hematology testing.  So,
> I guess I'm more a jack of all trades, rather than a master of this one.
> That is why I'm asking such a basic question.
> 
> When I worked at the Johns Hopkins Oncology Center, and did flow
> cytometric analyses of leukemias, we used leukemia cell lines (KG1a,
> Daudi, and MOLT3 cells) as positive controls for our leukemia antibodies.
>  The cells were supplied to us by one of the oncology research labs.
> Now I work at a community hospital that has a flow cytometry lab, and I
> no longer have access to these cell lines.  Does anybody know if there
> are stabilized leukemia cell lines that are sold commercially?   As you
> can see, they don't let me out of my cage too
> often.......................
> 
> Thanks very much in advance for any information you can give me.
> 
> Going with the flow,
> Terry Fecik
> Sinai Hospital of Baltimore



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