At 14:40 97 - 08 - 06 -0400, you wrote:
>
>Theresa,
>
>Try contacting the ATCC...sorry, don't have the address, but they are a
>bank of thousands of cell lines from which you can purchase.
>
>(A'flowin' just down the road from you!......)
>
>P. Echeagaray
>Flow Cytometry Services
>Southern Research Frederick
>Frederick MD
>echeagaray@sri.org
>
>Theresa L. Fecik wrote:
>>
>> Hi all,
>>
>> I'm a newbie here on this board--though, not necessarily a newbie in flow
>> cytometry. I have about 10 years experience in flow--but, I have also
>> been working in other areas of the lab at the same time, i.e.,
>> hematology, coagulation, urinalysis, and special hematology testing. So,
>> I guess I'm more a jack of all trades, rather than a master of this one.
>> That is why I'm asking such a basic question.
>>
>> When I worked at the Johns Hopkins Oncology Center, and did flow
>> cytometric analyses of leukemias, we used leukemia cell lines (KG1a,
>> Daudi, and MOLT3 cells) as positive controls for our leukemia antibodies.
>> The cells were supplied to us by one of the oncology research labs.
>> Now I work at a community hospital that has a flow cytometry lab, and I
>> no longer have access to these cell lines. Does anybody know if there
>> are stabilized leukemia cell lines that are sold commercially? As you
>> can see, they don't let me out of my cage too
>> often.......................
>>
>> Thanks very much in advance for any information you can give me.
>>
>> Going with the flow,
>> Terry Fecik
>> Sinai Hospital of Baltimore
>
Internet address of AMERICAN TYPE CULTURE COLLECTION :
http://www.atcc.org
Good luck,
wp
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Wladyslaw Pajdak
Dept. Clinical Immunochemistry, Skawinska 8, 31-066 Cracow, Poland
Int. Med. II, Collegium Medicum, Tel.: +48(12)56 54 25 ; +48(12)56 51 22
Jagiellonian University Fax: +48(12)56 57 86
E-mail:
mmpajdak@cyf-kr.edu.pl
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