Streptavidin Cy5

From: jonathan.welsh (jonathan.welsh@bbsrc.ac.uk)
Date: Thu Jul 31 1997 - 11:52:11 EST

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Hello all,

We are getting a lot of background fluorescence after using streptavidin Cy5 to
label biotinylated primary Mabs to mouse thymocyte surface markers. Can anyone
out there suggest a reason for this and how it can be minimised? Are there any
blocking steps that are needed for other biotinylated moieties on the cell
surface? Any thoughts would be much appreciated.

Thanks in advance,
Jon

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