As this journal is not available in our extensive library system at the University of California, could you perhaps hint at the protocol used? I have the Cytometry 8:23-239 (1987) paper by Beisker, Dolbeare and Gray, (and the Carnegie,J.A.,et al Journal of Histochemistry and Cytochemistry:244 pp308-310, 1980) but have been afraid to try the borohydride. Sigma tech support wasn't very encouraging. How long is the NaBH4 stable in PBS? Dennis ************************************************************************* * Dennis J. Young Voice : (619) 822-0407 * * Flow Cytometry Core Facility FAX : (619) 822-0412 * * University of California, San Diego USA e-mail: djyoung@ucsd.edu * * http://www-core.ucsd.edu/flow-core.html * ************************************************************************* ______________________________ Reply Separator _________________________________ Subject: Re: Autofluoorescence problems Author: Karenrt@AOL.COM at @UCSD Date: 7/27/97 4:47 PM There is a very recent article on this subject in the latest issue of Communic ations in Clinical Cytometry: Mosiman VL, Patterson BK, Canterero L, Goolsby CL. Reducing cellular autofluorescence in flow cytometry: an in situ method. Communications in Clinical Cytometry, Vol 30, No 3, 15 June 97, 151-156. Hope this helps! Karen Tamul, MS, MT(ASCP)SI Mid-Atlantic Sales Representative Caltag Laboratories Phone/Fax: (301) 540-3712 E-mail: karenrt@aol.com >-- Saved internet headers (useful for debugging) >Received: from flowcyt.cyto.purdue.edu by mail.ucsd.edu; id CAA04939 sendmail 8 >Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyt >Received: from emout08.mail.aol.com by flowcyt.cyto.purdue.edu via ESMTP (94081 >From: Karenrt@AOL.COM >Received: (from root@localhost) by emout08.mail.aol.com (8.7.6/8.7.3/AOL-2.0. >Date: Sun, 27 Jul 1997 16:47:15 -0400 (EDT) >Message-ID: <970727164713_2026137494@emout08.mail.aol.com> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >cc: cytometry@flowcyt.cyto.purdue.edu >Subject: Re: Autofluoorescence problems
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