i have had success with mouse bone marrow. the cell were first lineage depleted using primary antibodies and a dynabead secondary. the cells were then sorted for Sca1 positives by our very capable flow operator, kathy mckinnon (you're welcome kathy, you can mail me the check) on a FACS Vantage with TurboSort. i believe they were sorted at 30 psi. the Sca1+ cells were used in our soft agar colony cultures with excellent results. -brian white human genome sciences rockville, md brian_white@hgsi.com 301-309-8504 x2121
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