Hello, I have few information about viability after TSO sorting on FACSvantage : All sorts at 30 PSI drop drive 53100, drive level about 275, sample overpressure 2 PSI, about 20000 cells/sec. On human myeloma cell lines : regarding viability no real difference was seen. regarding colony forming unit on StemGem there was a small difference in number and size On CD34 presorted cells (CellPro or Baxter) and sorted with TSO only on CD34 positivity: regarding viability no difference was seen. regarding colony forming unit on StemGem no real difference was seen. On CD34 presorted cells (CellPro or Baxter) and sorted with TSO on CD34+CD38- basis : no difference was seen. regarding colony I have to do 2 or 3 other experiments but the first one was correct. DUPERRAY Christophe INSERM U291 99, rue Puech Villa 34197 MONTPELLIER cedex 5 Tel 33 (0)4 67 63 61 29 Fax 33 (0)4 67 04 18 63 Web site http://pythie.cnusc.fr:8101/
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