In a message dated 7/30/97 6:21:03 AM, you wrote: <<Subject: Lymph Node Dissociation Methods From: Bill Justice <bjustice@kumc.edu> at smtplink-nedh Date: 7/29/97 06:35 AM Hello I am asking this question on behalf of another flow cytometry operator at our institution. This is a very basic question. When processing lymph node for L/L panels, what are other labs doing to mechanically process the node? Tissue Homogenizers? scissors and scalpels? pushing through wire mesh? What? What are the pitfall and potential problems with difference techniques? Thanks. Bill Justice Flow Cytometry/PC Support KUMC Clinical Labs >> when i used to work with monkey tissues in a previous lab incarnation, we used the top ends of syringe plungers to mash up whole or pieces of lymph nodes and other organs in petri dishes. These were the10ml size with the ridged tops, not smooth. we then put the whole mush thru falcon 100um mesh filters for 50 ml tubes, rinsing it through with additional media. it worked well for flow analysis and culture. -brian white human genome sciences rockville, md brian_white@hgsi.com 301-309-8504 x2121
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