NOTE about postings: I am receiving many postings from colleagues who review this list on our website. I try to post them but I think that if people are interested enough to survey the web site, that in general they should decide to accept the email messages as everyone else. Some are new users who discover the EMAIL site and find it useful - these are the people that I put messages up for - others give the argument that they get too much email so they should be able to send messages, but not receive everyone elses. Remember the postings are not automatic like many others - that's why there is relatively little junk mail! My philosophy is that if you have to read them all, you will be careful what you send!! Sorry this diatribe has nothing to do with the following message which asks an important question!! ---------------------------------------------------------------------- The following message is posted for Virginia Cox <cox@medicine.tamu.edu>. Please reply to CYTOMETRY or to her address with a response. >I have been trying to sort E. coli with the GFP gene inserted for a >client. When we run the sorted bacteria through the machine to check for >purity, we see little fluorescence & the light scatter has changed. We >think we may be damaging the cells. Any experience or suggestions would >be appreciated. > --------------------------------------------------------------- Posted by > __________________________________________________________________ J.Paul Robinson, Ph.D., Professor of Immunopharmacology Director, Purdue University Cytometry Laboratories Purdue University Phone:(765)-4940757 FAX: (765)-4040517 EMAIL: robinson@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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