> Date: Mon, 21 Jul 97 12:34:35 -24000 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > From: Ralph Bohmer <ralph.bohmer@es.nemc.org> > Subject: fate of cultured progenitors > > > Could anyone help me find good references dealing the following subject: > > Proliferating hemopoietic progenitors in culture (semi-solid or liquid) > differentiate to a certain point, then they get stuck and apoptose, I guess, > even with all relevant cytokines present (?) > > How far do the cells from various lineages get ? > How do they die ? > How can death at this point be manipulated ? Ralph, You can answer a lot of these qustions by using cell surface labelling - see JEM 185 (7) 1337 and refs therein for analysis of erythroid cells. A few describing myeloid differentiation are listed below - there are many others: Kerst, J.M., Slapercortenbach, I.C.M., Vondemborne, A.E.G.K., Vanderschoot, C.E. and Vanoers, R.H.J. (1992) Combined measurement of growth and differentiation in suspension-cultures of purified human cd34- positive cells enables a detailed analysis of myelopoiesis. Exp. Hematol. 20, 1188-1193. Terstappen, L.W.M.M., Buescher, S., Nguyen, M. and Reading, C. (1992) Differentiation and maturation of growth-factor expanded human hematopoietic progenitors assessed by multidimensional flow-cytometry. Leukemia 6, 1001-1010. Scheinecker, C., Strobl, H., Fritsch, G., Csmarits, B., Krieger, O., Majdic, O. and Knapp, W. (1995) Granulomonocyte-associated lysosomal protein expression during in- vitro expansion and differentiation of cd34(+) hematopoietic progenitor cells. Blood 86, 4115-4123. ################################################################ From: Dr Richard Darley, Ph.D. LRF Differentiation Unit Department of Haematology (A7) University of Wales College of Medicine Heath Park Cardiff, CF4 4XN U.K. tel (+44) 12 22 74 34 85 or (+44) 12 22 74 45 24 Fax (+44) 12 22 74 45 23 e-mail: darley@cf.ac.uk
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