It is usually not necessary to wash out PI because bound PI has a much higher quantum efficiencey than free dye. Also, most flow cytometer electronics are designed to ignore constant, or DC background such as a fixed concentration of free dye in solution. gary Gary Durack University of Illinois Biotechnology Center voice (217) 244-0559 fax (217) 244-0466 durack@uiuc.edu http://www.life.uiuc.edu/biotech
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