RE: Semi-quantitation of CD4 receptors

From: Woo, Gary (gary@amgen.com)
Date: Wed Jul 09 1997 - 12:04:00 EST


hallo to you

you can try abe schwartz's simply cellular beads.  call Flow cytometry
standards corp.  800 227-8143.  they're fairly easy to use and you can
extrapolate the number of Ab bound to a cell (average of a population
actually)

hope this helps
gary woo

>----------
>From: 	Ras Smit
>Sent: 	Wednesday, July 9, 1997 8:59 AM
>To: 	Cytometry Mailing List
>Subject: 	Semi-quantitation of CD4 receptors
>
>
>Hallo everybody
>
>I believe a similar question has been recently asked, but can anybody 
>tell me how to determine the density of  CD4 receptors on lymphocytes
>with flow cytometry.  Any previous methods discussed on 
>this mailing list or references will be greatly appreciated.
>
>Ras Smit
>Dr EJ Smit; Registrar in Virology, Medical University of Southern Africa
>PO Box 173, MEDUNSA, 0204, South Africa
>Phone +27 12 521-4113  Fax +27 12 521-5794
>



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