I am posting this on behalf of the person listed below. Please reply directly to that person with comments. Thanks Paul ------------------------ Forwarded Message Follows - - - - - - - Hi- I hope this is the proper route to post something on the archive. I've tracked down several protocols for propidium staining, but I do have one question they do not address. Don't you need to centrifuge the stained samples before running them through the flow cytometer? I would imagine that you still have some PI in solution, and that it would give you an awfully high background signal. Any comments? Thanks. This is a wonderful forum. Bob Crow crow@cbnmr.tamu.edu ---------------------------------- posted by J.Paul Robinson, Purdue University Cytometry Labs Professor of Immunopharmacology robinson@flowcyt.cyto.purdue.edu PH:765-494 6449 FAX:765-494 0517 web http://www.cyto.purdue.edu
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