Dear Chang Seok, We often do T-cell lymphocyte subset analysis after Cd34 collection. The reason being that most of our allogeneic transplants are T-cell depleted. We however use Campath 1 which often depletes other cell lines as well. In our experience there is a huge drop in the T-cell population but we have not seen the huge increase in CD19+ cells that you have seen. We also haven't experienced the CD3+/CD19+ population you describe. I hope this has been helpful. I would be interested to hear what others have experienced. Good luck, Glenda Davison Dept.Haematology Groote Schuur Hospital University of Cape Town South Africa email: gmd@samiot.uct.ac.za
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