Chang-Seok, I've done a couple CD34 selections on G-CSF mobilized normal-donors with the Baxter ISOLEX system and have never experienced such results. I've observed that the non-target cells that are captured are primarily monocytes (CD14+). I almost never see any CD19+ positive events. It's common to see CD34+CD19+ events in the bone marrow but I've never observed these cells mobilized to the peripheral blood. Aftter G-CSF, most are CD34+CD33+. What was the CD34% of the product? Were these cells also CD45dim? Were all the events in the selected fraction "lymph-sized" based on scatter? What was the circulating peripheral CD34/ul count? Drop me a note at tfarley@computer.net Maybe I can be of some help Dr. Timothy Farley New York Blood Center Clinical Services Valhalla, NY
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