We are using Propidium Iodide to stain the DNA of fish and chicken blood cells and then using the fish/chicken emission ratios to calculate the amount of DNA in the fish cells. All this is being done on a Becton-Dickinson FACScan with Cellfit software. We have noticed that the FL2H (585 +/- 21 nm bandpass) and the FL3H (650 nm longpass) fish/chicken ratios give us different answers. FL3H consistently gives us a lower ratio by 5-10%. Shouldn't they give us the same ratio?
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