On Fri, 27 Jun 1997, Stacie Anderson wrote: > > We are interested in sorting viable human monocytes (CD14+) to be used in > functional cytokine production assays. We have done the experiment once > with poor monocyte viability. The monocytes were sorted, placed into > culture for 24 hours and then stimulated with LPS and monensin. The > combination of these events killed the monocytes - any suggestions? Thanks > in advance for any suggestions. Stacie Anderson > > Stacie Anderson > Laboratory of Gene Transfer, Hematopoeisis Section > NCHGR, NIH > Room 2C12 > 49 Convent Drive > Bethesda, MD 20892-4442 > > > Try sorting into 5 ml polypropelene tubes with 1/2 ml HI/FCS in the tube. Keep the collected cells cold!!! You can also try sorting into a petri dish with HI/FCS. Jim
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