We are interested in sorting viable human monocytes (CD14+) to be used in functional cytokine production assays. We have done the experiment once with poor monocyte viability. The monocytes were sorted, placed into culture for 24 hours and then stimulated with LPS and monensin. The combination of these events killed the monocytes - any suggestions? Thanks in advance for any suggestions. Stacie Anderson Stacie Anderson Laboratory of Gene Transfer, Hematopoeisis Section NCHGR, NIH Room 2C12 49 Convent Drive Bethesda, MD 20892-4442
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