On 25 Jun 97 at 22:38, Bob Ashcroft wrote: > Is the He-Ne path of higher wavelength excitation superior to the > He-Cd (or Hg arc) path of shorter wavelength excitation? > Can you give me your tuppence-worth on this? Generally sppekaing, red excitation has an advantage over UV and even 488 nm excitation in that there is less autofluorescence from the biological material that you are trying to study. One of the exceptions to this rule is plant material of course. Obviously the best and most flexible path is for new dyes to be developed for all of the wavelengths that we would wish to use. I'm not convinced that UV has been overlooked in favour of red excitaion but that may be because I'm looking around desperately for more red dyes to use with a laser diode-based instrument at the moment... > Can you give me your tuppence-worth on this? Well in local currency that's my 2c (ceiniog, not cents of course!) Haze ---------------------------------------------------------- | Hazel Marie Davey hlr@aber.ac.uk | |Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences| |Prifysgol Cymru * University of Wales| | ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DA | | http://pcfcij.dbs.aber.ac.uk/index.htm |
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