Dear Colleagues, PREAMBLE: In the wavelength continuum for excitation of fluorochromes, 488nm is the Everest. In the quest for extra colours, we can add tandems for 488-excitation, or we can add light sources which move to higher or to lower wavelengths of excitation. But which way is better? Is the He-Ne path of higher wavelength excitation superior to the He-Cd (or Hg arc) path of shorter wavelength excitation? The weight of activity definitely vindicates 488 tandems and He-Ne, but why not UV conjugates like AMCA and tandems for them? Why not viloet-excited conjugates? Was not the choice driven by what was easy at that time, and should we not now review it? THE QUESTION I AM PUTTING: IS CONJUGATE-SPACE SYMMETRIC, OR IS IT BETTER RED THAN DEAD-violet,..-indigo,...blue?? Have we given UV and violet a fair go in the antibody-fluorochrome conjugate challenge? What are the fluorochromes which are suitable conjugates for monoclonal antibodies, and which excite in the violet or the UV? Can you give me your tuppence-worth on this? ....still wondering,... Bob
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