My problem is this:During some sorts I get ,what I will call a spurious extra drop. To explain more,there are times I sort to polycarb filters.When all is well I get a single spot which contains the desired cell population.When all is supposed not well I get my desired single spot with cells and just about a quarter of inch from the desired spot I observe a spurious spot,which does not contain the desired cells.I maintain the nozzle on a strict PM cleaning schedule and watch for clogs etc.during times of sorting.The drive frequency remains constant during a sort and is generally consistent day to day.The amplitude level requires adjustment during a sort run.Should I be suspect that the amplitude level is giving the problem? Can anyone suggest other potential problem areas.Can anyone give rules/guidelines they follow when establishing drop drive frequency and amplitude level?Thanks.
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