There was a session at the last ISAC meeating in Remini dealing with some of these questions. It was chaired by Dr. Marvin_Meistrich. Is it possible to use FITC oligos to specific regions of the Y >>chromosome to accomplish this? I have sent the graduate student back to >>search >>out more on this possibility, but am wondering if anyone out there is already >>doing this or has found it to be impossible, or is using another even more >>elegant method? > >I doubt that you could get the oligos to hybridize while retaining >viability. I keep hearing that there are X- and/or Y-specific surface >antigens, which would be ideal. Larry Johnson of the U. S. Department of >Agriculture probably knows more about sex selection by sperm sorting than >anybody else. Some references from a few years back are: > >Cran DG, Johnson LA, Miller NG, Cochrane D, Polge C: Production of bovine >calves following separation of X- and Y-chromosome bearing sperm and in >vitro fertilisation. Vet Rec 132:40-1, 1993 > >Hendriksen PJ, Tieman M, Van der Lende T, Johnson LA: Binding of anti-H-Y >monoclonal antibodies to separated X and Y chromosome-bearing porcine and >bovine sperm. Mol Reprod Dev 35:189-96, 1993 > >-Howard
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:51 EST