Greetings, everyone: I am in need of a method to block Fc receptors on human cells. We have antibodies (whole molecule) against a ligand on the surface of certain cells that we want to use to block binding of those cells to their target - a receptor on a human macrophage cell line. However, we need to prevent binding to the Fc receptors on the macrophages via the Fc portion of our antibodies. We prefer not to have to make F(ab')2 fragments of all of the antibodies we will be testing. I know that anti-CD16/32 monoclonals are available to block Fc receptors in the mouse, but as far as I have been able to find out, the anti-human CD16 and CD32 monoclonals are not efficient as blocking agents. If you have an efficient method of blocking human Fc receptors, please let me know. And, thanks in advance.
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