Frederic, As far as I know you can only use either FL4 A+W OR FL4H, you can't get FL4 H+W+A on a FACScalibur 4 Color machine. Neither can you use FL1,2,3 A+W both (only FL1,2,3, A, not W) if you activate FL4H. I think it's probably because the 4 Color option was designed as an upgrade and had to fit into existing FACSsort circuitry... I don't know the exact technical details (maybe somebody from BDIS could explain them...), but your are not missing something neither did you find a bug in the software, things are designed like this... but maybe if we make some noise they'll realise that this is a badly missing feature and change it in a future version. Hope that helps... Cheers, Matthias At 10:37 -0400 16/06/97, Frederic Preffer wrote: >With doublet discrimination option 'on', on FL4, using CellQuest software, >is it possible to get FL4 height? We cannot and are only getting FL4 width >and area. We would like to collect FL1,2, & 3 along with all three of these >FL4 parameters. Is this a 'bug' or missetting in our software, or something >it is not designed to do?
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