I am having some problems in setting up the FacStar+ for 4-color flow using FITC/PE/Tricolor/APC with argon and HeNe lasers and a 650LP filter. We have been having considerable trouble detecting a Tricolor signal, although a good signal can be detected using the same sample on our FacScan. We have used the same antibody in biotinylated form with a PE/Cychrome secondary (also on the FacStar+) with good results, but this does not solve our problem as we need to use the biotin for the SA-APC combination. Has anyone else experienced this problem using the Tricolor? Any advice on instrument set-up or compensation would be greatly appreciated. Darlene Steele-Norwood McMaster University Department of Medicine Hamilton, Ontario
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