Re: adherant cells

From: Mikael Vestberg (mikael.vestberg@inflam.lu.se)
Date: Fri Jun 13 1997 - 06:05:41 EST


>Could anyone suggest a method of removing adherant cell lines from culture
>vessels which does not remove surface adhesion molecules? At present i am
>studying surface cell adhesion molecules expression on human umbilical vein
>endothelial cells using flow cytometry.
>i would appreciate any suggestions.
>thanks
>Morgan


Try cell dissociation medium from Sigma. It works fine on endothelial cells
and is supposed to be protease free


Mikael



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