We do this all the time. If you can, try using a CaMg-free incubation; we use Tyrode's. 10-15 min incubation in incubator, change it twice, then rinse the cells off. If they don't come off, try a very short (10-20 sec) exposure to trypsin or trypsin/EDTA, then rinse off with culture medium. Get back to me if this doesn't work. Deb Berglund Montana State University On Thu, 12 Jun 1997 ans033@abdn.ac.uk wrote: > > Could anyone suggest a method of removing adherant cell lines from culture > vessels which does not remove surface adhesion molecules? At present i am > studying surface cell adhesion molecules expression on human umbilical vein > endothelial cells using flow cytometry. > i would appreciate any suggestions. > thanks > Morgan >
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